When we amplified the heavy-chain variable regions (VH) gene from one hybridoma cell line which were not amplified successfully under conventional protocols, a novel method was developed to design the ...

ImmuMab Heavy ® mice are able to produce fully human antibodies, consisting solely of heavy chains, without the presence of light chains. These mice possess over 120 human immunoglobulin heavy-chain V ...

Researchers isolated SARS-CoV-2 spike protein-targeted mAbs from convalescent healthcare workers, emphasizing the IGHV1-69 gene, the gene with the greatest structural and allelic variation.

Both groups also retained some other essential processes, such as segregated and sequential rearrangement of heavy and light chain loci, preferential rearrangement of light chain kappa before lambda, ...

Conventionally, SHM testing has been performed using labor intensive and primarily analog Sanger sequencing method following PCR amplification of the clonal immunoglobulin heavy chain gene ...

The B-1a cell heavy chain antibody repertoire differs dramatically from the follicular and marginal zone B cell repertoire and is defined by distinct mechanisms driven by self antigens rather than ...

B-lymphocyte development is dictated by the protein products of functionally rearranged Ig heavy (H) and light (L) chain genes. Ig rearrangement begins in pro-B cells at the IgH locus. If pro-B cells ...