When in close proximity, the quencher will effectively block the fluorescence signal emission from the fluorophore. However, the 5´ to 3´ exonuclease activity of the DNA polymerase enzyme during ...

The focused 9n DNA polymerase library was generated by replacing ... a template with a fluorophore label at the 5′ end and a quencher probe labelled with a quencher dye at the 3′ end.

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction ... The fluorescent reporter (R) is shown in green and the quencher (Q) in purple, target DNA in grey, amplified ...

The researchers eventually found a polymerase from Bacillus subtilis that was ... Researchers use probes with a fluorophore and a quencher attached by single-stranded DNA, so when the target DNA is ...

Quantitative polymerase chain reaction (qPCR ... qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from ...

Analysts interested in quantifying and verifying the presence of DNA or RNA specific to an organism in a sample should consider using the Thermo Fisher QuantStudio 3 system. The QuantStudio system 3 ...

At the heart of this method lies the Bst DNA polymerase, whose enzymatic properties—such as strand displacement activity, thermostability, and catalytic efficiency—are critical to the success ...