However, the 5´ to 3´ exonuclease activity of the DNA polymerase enzyme during the elongation phase of each PCR cycle will digest the 5´ end of the probe, separating it from the quencher ...

To test the Cy3-Iowa Black FQ donor–quencher pair in a PAA, the RNA synthesis activity of an engineered DNA polymerase was compared with its wild-type (wt) DNA polymerase counterpart.

The DNA polymerase’s 5’ exonuclease activity cleaves the reporter, separating it from the quencher and emitting fluorescence ; The probes are highly specific but require precise and custom design.

Recombinase polymerase amplification (RPA) is a technique for rapidly copying segments of DNA. Unlike polymerase chain reaction (PCR), it does not require thermal cycling, ... Researchers use probes ...

Analysts interested in quantifying and verifying the presence of DNA or RNA specific to an organism in a sample should consider using the Thermo Fisher QuantStudio 3 system. The QuantStudio system 3 ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

Double-Quenched Probes Designed to Be Stable at High Temperatures and Minimize Fluorescence Within the field of molecular biology, quantitative polymerase chain reaction (qPCR) processes enable ...