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Competitive PCR–ELISA protocols for the quantitative and the standardized detection of viral genomes
PCR–ELISA can be performed in 1 d in laboratories ... of a single known amount of competitor to amplification reactions. Such a competitor can even be added to the original sample to act as ...
Real-time PCR shows similar sensitivity to the PCR–ELISA, but has the advantage of being a sealed system, decreasing the risk of carryover contamination during the reaction, which can be a ...
Lei introduced a two-step amplification to the ELISA reaction. Lei first added a tyramide signal amplification (TSA) process to amplify the signal of a low abundance biomarker. In Lei's platform ...
An 82-year-old on haemodialysis tests HIV-positive 7 months in, only for PCR to be negative, implicating flu shot antibody ...
Hybridization-based enzyme-linked immunosorbent assays (hybridization-based ELISA). Quantitative polymerase chain reaction (qPCR). Different analytical methods have varying requirements, advantages, ...
ELISA is a common immunoassays used for HCP detection. ELISA stands for “enzyme-linked immunosorbent assay” and is based on using polyclonal antibodies that have been raised against the host ...
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