introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, including off-target cutting. Over the years, scientists have ...

However, when it comes to inserting exogenous DNA using homology-directed repair mechanisms, CRISPR editing can be woefully inefficient. Yet, inserting DNA is crucial to engineering CAR T cells.

For these systems to work, the CRISPR payloads must pack a sequence encoding a gRNA for precise DNA targeting, instructions for one or more Cas effectors for making the genome breaks, and repair ...

Genome editing with various CRISPR-Cas molecule complexes ... The latter requires a copyable template for repair to accurately rejoin the DNA at the cut site. The slow variant is called homology ...

that can repair any damage caused by CRISPR-Cas systems. Defense systems that the bacteria uses to protect itself from foreign DNA often co-localize within defense islands (genomic segments that ...

It’s a new way to create “bi-paternal” mice that can survive to adulthood—but human applications are still a long way off.

The precise insertion of DNA sequences using the homology-directed repair (HDR) pathway is one potential use of the CRISPR/Cas genome editing technology. Various circumstances could influence the ...

Results prove that inversions can be generated in plants without causing further unwanted changes in the expression of genetic information.

This illustration compares various gene editing technologies, including meganucleases, zinc finger nucleases, TALENs, and CRISPR/Cas9, along with their relative feasibility and mechanisms of DNA ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Mutations in the BRCA2 gene can prevent DNA repair and thus predispose carriers to malignancies ... informs the clinical management of hereditary cancers. The study used CRISPR-Cas9 technology, a ...