Genome editing technologies like CRISPR-Cas9 have transformed biology, medicine, and agriculture, but concerns remain about ...

Your DNA is continually damaged by sources both inside and outside your body. One especially severe form of damage called a double-strand break involves the severing of both strands of the DNA double ...

For decades, scientists have studied how to control DNA—the molecule that holds the instructions for all life. One of the most powerful tools to emerge from that effort was CRISPR, a system adapted ...

Cells are constantly subjected to DNA damage from a range of internal and environmental sources. It is estimated that cells ...

Reference: Yuan B, Bi C, Tian Y, et al. Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA ...

The investigators used CRISPR along with an enzyme called Cas13. While this enzyme can cut DNA, the researchers altered it. “Cas13 naturally acts like a pair of scissors, but we engineered it to act ...

In the recent studies, scientists describe jumping gene systems that, in a process the teams are alternatively calling bridge editing and seekRNA, can be modified to cut, paste, and flip any DNA ...

Once a cut is made, it is a multistep, imperfect process of cellular DNA repair. CRISPR can be used to make other types of edits too, such as increasing or decreasing the effects of a gene ...

Avoiding CRISPR Off-Target Effects by Tweaking the Timing of Edits. Double-strand DNA breaks not only open the door to unwanted, off-target editing, but their improper repair can lead to additional ...